Phenolbụ molekul nke na-arụ ọrụ dị mkpa n'ọtụtụ mmeghachi omume kemịkalụ ma jiri ya mee ihe n'ọtụtụ ngwa ọrụ mmepụta ihe. Ya mere, ọ dị mkpa ịnweta usoro a pụrụ ịdabere na ya iji chọpụta phenol n'ụdị dị iche iche. N'isiokwu a, anyị ga-enyocha usoro dị iche iche dị iche iche iji chọpụta phenol, uru na ọghọm ha, na mkpa nke njirimara phenol na ndụ kwa ụbọchị na ụlọ ọrụ.

1. Gas Chromatography (GC)

Gas chromatography bụ usoro nyocha nke a na-ejikarị eme ihe maka ịchọpụta phenol. N'ime usoro a, a na-agbanye ihe nlele ahụ n'ime kọlụm jupụtara na nkeji oge. Usoro mkpanaka wee na-agafe na kọlụm ahụ, na-ekewapụta akụkụ nke ihe nlele ahụ. Nkewa ahụ dabere n'ụdị solubility nke ihe ndị dị n'ụdị ọdụ na mkpanaka.

Uru: GC nwere mmetụta nke ukwuu, akọwapụtara nke ọma na ngwa ngwa. Ọ nwere ike ịchọpụta obere mkpokọta phenol.

Ihe ọghọm: GC chọrọ ndị ọrụ a zụrụ azụ nke ukwuu na akụrụngwa dị oke ọnụ, na-eme ka ọ ghara ịdị mma maka nnwale ubi.

2. Chromatography Liquid (LC)

chromatography nke mmiri dị ka chromatography gas, mana a na-etinye oge a na-akwụsị n'ime kọlụm kama ịbụ nke mkpuchi ya na nkwado kwụ ọtọ. A na-ejikarị LC maka ikewa nnukwu ụmụ irighiri ihe, dị ka protein na peptides.

Uru: LC nwere oke nkewa arụmọrụ ma nwee ike ijikwa nnukwu ụmụ irighiri ihe.

Ọdịmma: LC adịghị enwe mmetụta karịa GC ma na-achọkwu oge iji nweta nsonaazụ.

3. Spectroscopy

Spectroscopy bụ usoro na-adịghị emebi emebi nke gụnyere ịlele nnabata ma ọ bụ mgbapụta radieshon site na atọm ma ọ bụ ụmụ irighiri ihe. N'ihe gbasara phenol, a na-ejikarị infrared spectroscopy na resonance resonance nuklia (NMR) spectroscopy eme ihe. Infrared spectroscopy na-atụle nnabata nke radieshon infrared site na ụmụ irighiri ihe, ebe NMR spectroscopy na-atụkwa nnabata nke radieshon redio site na nuclei nke atọm.

Uru: Spectroscopy bụ nke ukwuu kpọmkwem ma nwee ike ịnye ozi zuru ezu gbasara nhazi nke ụmụ irighiri ihe.

Ihe ọghọm: Spectroscopy na-achọkarị akụrụngwa dị oke ọnụ ma nwee ike iwe oge.

4. Ụzọ agba agba

Ụzọ agba na-agụnye ime ihe nlele na reagent iji mepụta ngwaahịa nwere agba nke enwere ike tụọ spectrophotometrically. Otu usoro colorimetric a na-ahụkarị maka ịchọpụta phenol gụnyere imeghachi ihe nlele ahụ na 4-aminoantipyrine n'ihu ihe njikọ njikọ iji mepụta ngwaahịa na-acha ọbara ọbara. Ike nke agba na-adabere kpọmkwem na ntinye nke phenol na nlele.

Uru: Ụzọ Colorimetric dị mfe, adịghị ọnụ, enwere ike iji ya mee nyocha ubi.

Ọdịmma: Ụzọ Colorimetric nwere ike ọ gaghị achọpụta ụdị phenol niile.

5. Nnwale ndu

Nnwale ndu N'iji mmeghachi omume physiological akọwapụtara nke ihe dị ndụ iji chọpụta ọnụnọ, akụrụngwa na ọdịnaya nke ihe ebumnuche. Dịka ọmụmaatụ, ụfọdụ nje bacteria na yists nwere ike ịtụgharị phenol ka ọ bụrụ ngwaahịa nwere agba nke enwere ike tụọ spectrophotometrically. Nnwale ndị a akọwapụtara nke ukwuu mana ọ nwere ike ọ nweghị mmetụta na obere mkpokọta.

Uru: Nnwale ndu bụ nke akọwapụtara nke ọma ma enwere ike iji ya mee nchọpụta ogige ọhụrụ.

Ọdịmma: Nnwale ndu nwere ike ghara inwe uche ma na-ewekarị oge.